Immunoprecipitation Guide

Immunoprecipitation (IP) is a method of precipitating target protein out of solution by using an antibody that specifically binds to that particular protein. This process is powerful to isolate and concentrate a particular protein and/or protein complex from a sample containing many thousands of different proteins.
Individual protein immunoprecipitation is a procedure by using an antibody that is specific for a known protein to isolate that particular protein out of a solution containing many different proteins. It can also be used to “enrich” a protein population prior to Western Blotting.
Co-immunoprecipitation is an extension of IP that is immunoprecipitation of intact protein complexes. By targeting a known member of the protein complex with an antibody, co-IP makes it possible to pull the entire protein complex out of solution and thereby identify unknown binding partners and the function of the target protein.

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