Introduction
PBS: To prepare 1 L 1X PBS: dissolve 8g of NaCl, 0.2g of KCl, 1.44g of Na2HPO4, 0.24g of KH2PO4 in 800ml distilled H2O, then adjust pH to 7.4 with HCl and adjust volume to 1L with additional distilled H2O.
Fixation solution: 4% Paraformaldehyde, in PBS.
Permeablization solution: 0.3% Triton X-100 in PBS. To prepare 100 mL: add 300 µl Triton X-100 to 100 mL PBS and mix. (Optional)
Blocking buffer: To prepare 10 ml, add 0.5 ml normal serum in PBS and mix well. While stirring, add 30 µl Triton™ X-100.
NOTE: Select serum that are from the same species as the secondary antibody.
Antibody dilution buffer: To prepare 10 ml, add 30 µl Triton X-100 to 10 ml 1X PBS. Mix well then add 0.1g BSA.
Antifade Reagent
Fixation solution: 4% Paraformaldehyde, in PBS.
Permeablization solution: 0.3% Triton X-100 in PBS. To prepare 100 mL: add 300 µl Triton X-100 to 100 mL PBS and mix. (Optional)
Blocking buffer: To prepare 10 ml, add 0.5 ml normal serum in PBS and mix well. While stirring, add 30 µl Triton™ X-100.
NOTE: Select serum that are from the same species as the secondary antibody.
Antibody dilution buffer: To prepare 10 ml, add 30 µl Triton X-100 to 10 ml 1X PBS. Mix well then add 0.1g BSA.
Antifade Reagent
Contact Info
Address:
45-1 Ramsey Road, Shirley, NY 11967, USA
Shirley
11967
United States
Shirley
11967
United States
Tel:
Website:
https://www.creative-diagnostics.com/immunofluorescence-protocol-cultured-cell.htm
Hours of operation
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| Monday | Open | ||||||
| Tuesday | Open | ||||||
| Wednesday | Open | ||||||
| Thursday | Open | ||||||
| Friday | Open | ||||||
| Saturday | Open | ||||||
| Sunday | Open | ||||||
