Stable Cell Line
11 RESULTS
NY, NY
Tel: 6316197922
Since CRISPR/Cas nuclease was first introduced into eukaryotes it shortly becomes the most widely used tool for genome engineering. Either coding region or non-coding region can be delete by genome-editing tools. CRISPR/Cas9 targets Cas9 nuclease to specific DNA sequence by guide RNA and introduces double strand break. Repair of the DSBs may result in indels which lead to frame shift of gene and disrupt gene expression at endogenous level. In most cases frame shift is sufficient to block function of genes. However a single site of gene editing may be not sufficient to disrupt function when targeting to microRNA or... read more
Tel: 6316197922
Since CRISPR/Cas nuclease was first introduced into eukaryotes it shortly becomes the most widely used tool for genome engineering. Either coding region or non-coding region can be delete by genome-editing tools. CRISPR/Cas9 targets Cas9 nuclease to specific DNA sequence by guide RNA and introduces double strand break. Repair of the DSBs may result in indels which lead to frame shift of gene and disrupt gene expression at endogenous level. In most cases frame shift is sufficient to block function of genes. However a single site of gene editing may be not sufficient to disrupt function when targeting to microRNA or... read more
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