Fragment Deleted Stable Cell Line

Since CRISPR/Cas nuclease was first introduced into eukaryotes, it shortly becomes the most widely used tool for genome engineering. Either coding region or non-coding region can be delete by genome-editing tools. CRISPR/Cas9 targets Cas9 nuclease to specific DNA sequence by guide RNA and introduces double strand break. Repair of the DSBs may result in indels, which lead to frame shift of gene and disrupt gene expression at endogenous level. In most cases, frame shift is sufficient to block function of genes. However, a single site of gene editing may be not sufficient to disrupt function when targeting to microRNA or other non-coding region. To overcome this limitation, paired guide RNA targeting both 5’ and 3’ of microRNA are introduced into cells simultaneously for whole region deletion. The large fragment deletion disrupts the structure of miRNA and then results in functional knockout. This strategy also applies to other region of genome, like lncRNA, promoter and enhancer.

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Monday	Open	9:00am	5:00pm
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Wednesday	Open	9:00am	5:00pm
Thursday	Open	9:00am	5:00pm
Friday	Open	9:00am	5:00pm
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Sunday	Open