surface plasmon resonance imaging

Surface Plasmon Resonance imaging (SPRi), namely surface plasmon resonance microscopy (SPRM), is a real-time, label-free, and high-throughput technique which is used to study biomolecular interactions based on detecting the refractive index changes resulting from molecular binding. More specifically, SPRi has been developed to examine affinity between biomoleculars, screen biomarkers and detect biopsy specimens.

Applications:

Kinetic characterization by Surface Plasmon Resonance and other methods has already been an important step for drug researchers to select and characterize novel therapeutics as well as for basic life scientists to investigate specific biological binding events. When combined with appropriate surface chemistry, microfluidics and software, SPRi is unmatched in its range of applications including:

Affinity analysis
Kinetic analysis
Ligand-fishing
Epitope mapping
Protein / protein interaction
DNA/DNA interactions
Protein/carbohydrates
Protein / DNA interactions
RNA/RNA interactions
Antibody (protein) / bacteria interactions
Antibody / cell interactions
Peptide / protein interactions
Polymer / polymer interactions

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