Introduction
Creative Biolabs is one of the well-recognized experts who is professional in applying advanced phage display technologies for a broad range of project objectives. With years of experience, our scientists can offer high-quality phage display library construction and custom phage display library screening services to meet our clients’ demands precisely. Particularly, our services also involve specific antibody discovery (e.g. PTM-specific antibody, anti-idiotype antibody, and agonistic antibody) and peptidome discovery.
Library Construction and Screening
Library Construction and Screening
Fig.1 Workflow of phage display construction
and screening (DÃez et al. 2015)
Phage display is one of the most powerful and widely used laboratory technique for the study of protein-protein, protein-peptide and protein-DNA interactions. This technology is mainly based on displaying the interest protein (peptides, antibodies, scaffolds or others) on the surface of employing phage and then be used to interrogate the constructed libraries containing millions or even billions of displayed phages. Theoretically, phage display is an exogenous gene expression method which the gene encoding the interest protein is inserted into bacteriophage coat protein gene then displaying the interest protein on the phage surfaces, resulting in a connection between genotype and phenotype.
Due to the different properties and advantages, Creative Biolabs is pleased to tailor the most appropriate phage display system (M13, T4 or T7) to meet our customers’ demands. Through DNA manipulation, numerous gene variants can be created and constructed as phage display library. Our scientists are confident in generating high-quality libraries with the diversity of 108. Furthermore, to detect the interaction between displayed protein and those other molecules or isolate specific target binders, Creative Biolabs also utilizes the novel biopanning strategies to select high-affinity binders or ligands which are able to recognize the naïve targets.
Library Construction and Screening
Library Construction and Screening
Fig.1 Workflow of phage display construction
and screening (DÃez et al. 2015)
Phage display is one of the most powerful and widely used laboratory technique for the study of protein-protein, protein-peptide and protein-DNA interactions. This technology is mainly based on displaying the interest protein (peptides, antibodies, scaffolds or others) on the surface of employing phage and then be used to interrogate the constructed libraries containing millions or even billions of displayed phages. Theoretically, phage display is an exogenous gene expression method which the gene encoding the interest protein is inserted into bacteriophage coat protein gene then displaying the interest protein on the phage surfaces, resulting in a connection between genotype and phenotype.
Due to the different properties and advantages, Creative Biolabs is pleased to tailor the most appropriate phage display system (M13, T4 or T7) to meet our customers’ demands. Through DNA manipulation, numerous gene variants can be created and constructed as phage display library. Our scientists are confident in generating high-quality libraries with the diversity of 108. Furthermore, to detect the interaction between displayed protein and those other molecules or isolate specific target binders, Creative Biolabs also utilizes the novel biopanning strategies to select high-affinity binders or ligands which are able to recognize the naïve targets.
Contact Info
Address:
45-1 Ramsey Road, Shirley, NY 11967, USA
Abingdon
Illinois 11967
United States
Abingdon
Illinois 11967
United States
Tel:
Website:
https://www.creative-biolabs.com/phage-display-service.html
Hours of operation
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| Monday | Open | ||||||
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| Wednesday | Open | ||||||
| Thursday | Open | ||||||
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| Sunday | Open | ||||||
