peptide mapping

Proteomics is the largescale study of proteins, including protein, protein complexes, protein signaling networks, protein structures and functions, etc. One of its most important tasks of proteomics study is to develop efficient and rapid approaches to identify various proteins. Peptide mapping is a efficient method to rapidly map the protein sequence, and thus is commonly used strategy in protein identification. Peptide mapping is also an important technique for investigating protein primary structures and determining surface-exposed sites or epitopes within proteins. Prior to peptide mapping, the proteins and peptides usually need to be separated and purified by a variety of separation approaches, such as gel electrophoresis, liquid chromatograph, capillary electrophoresis, etc. Then proteins are digested into peptide fragments. These resultant fragments are subsequently identified by mass spectrometry to obtain peptide mass fingerprints.

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