Introduction
In a general “a-agglutinin†display system, the protein of interest will be flanked by two epitope tags: a 9-amino acid hemagglutinin antigen (HA) tag and a 10-amino acid c-myc tag, and then will be fused to the C-terminus of the a-agglutinin Aga2p subunit. Following translation, the 69-amino acid Aga2p subunit associates with 725-amino acid a-agglutinin Aga1p subunit via two disulfide bonds. The fusion protein is subsequently secreted to the extracellular space where Aga1p is anchored to the cell wall via a β1,6-glucan covalent linkage. As a result, the protein of interest is displayed on the cell surface where it is accessible by soluble ligands (as shown below).
Yeast is superior to phage for human antibody display in that the eukaryotic expression environment of yeast cells is more suitable for human protein folding, modification, and translocation for display. Therefore, the yeast displayed antibodies are conformationally and functionally closer to human native counterparts.
Yeast is superior to phage for human antibody display in that the eukaryotic expression environment of yeast cells is more suitable for human protein folding, modification, and translocation for display. Therefore, the yeast displayed antibodies are conformationally and functionally closer to human native counterparts.
Contact Info
Address:
45-1 Ramsey Road, Shirley, NY 11967, USA
Shirley
Illinois 11967
United States
Shirley
Illinois 11967
United States
Tel:
Website:
https://www.creative-biolabs.com/yeast-display-services.html
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