bound antibodies

Creative Biolabs utilizes target antibodies in scFv forms or using peptides designed according to the sequences of the variable domains as targets to isolate anti-idiotypic antibodies from immunized phage display library. If variable regions of both the heavy- and light-chains contribute idiotypic determinants, the idiotype is likely to be a conformational determinant, then scFv form will be used. On the other hand, individual idiotypes may be located either in the heavy- or light-chains and contain the primary amino acid sequences of the variable regions. These idiotypes are more likely to be sequence-dependent, and linear antigenic determinants. In this situation peptides will be utilized as target proteins. Anti-ID antibodies can then be isolated by immunized phage display library technology.

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