Protein-RNA Interaction Analysis

CLIP (cross-linking immunoprecipitation) is a method used to analyze RNA-protein interactions or locate precise RNA modifications. This technique is performed by immunoprecipitation with an antibody against the protein-of-interest after UV cross-linking of the RNA-protein complex. CLIP-Seq, which is also known as HITS-CLIP, combines CLIP with high-throughput sequencing for identification of binding sites on RBPs. CLIP-Seq can be used to map RNA-binding landscape of interacting proteins or RNA modification sites on a genome-wide scale, which helps facilitate the understanding towards post-transcriptional regulatory networks and mechanisms.

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