Lateral Flow Assay

Lateral flow assay is a new in vitro diagnostic technique developed on the basis of monoclonal antibody technology, colloidal gold immunochromatography and new material technology in the 1990s, with rapid, simple, single detection, low-cost features. It is now widely used in medical test, food safety, environmental, agricultural and veterinary safety, inspection and quarantine, forensic fields, etc.The specific antigen or antibody was immobilized on the NC membrane (nitrocellulose membrane) with a large pore size microporous membrane as a carrier. When the sample was added to the sample at one end of the test strip, on the adsorbent sample pad, it had specific immune response with the binding of colloidal gold or microspheres labeled reagent via lateral movement by capillary action; then moved to the NC membrane, and then captured by antigen or antibody fixed in the NC membrane surface, and gathered in the detection area, then the density of the light-reflecting signal was visually obtained by visualizing the nitrocellulose surface marker (colloidal gold or latex particles). Other unbounded markers crossed the test strip and flow into the absorbent pad to achieve the purpose of automatic separation.

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