Knockout Cell Line Generation

Gene knockout cell line is a useful tool for research and manufacture. But it takes a long time to generate a knockout cell line. The most common method for gene editing is to use engineered nucleases, which can induce a double-strand break in genomic DNA. The induced double strand breaks are repaired through NHEJ or HR pathway, resulting in targeted mutations. To date, there are three widespread engineered nucleases for gene editing, ZFNs, TALENs and CRISPR/Cas9. As the latest technology, CRISPR/Cas9 are widely accepted for gene knockout and knockin. In CRISPR/Cas9 system, there are two key factors, Cas9 enzyme and guide RNA.

		From	To	From	To	From	To
Monday	Open	9:00am	5:00pm
Tuesday	Open	9:00am	5:00pm
Wednesday	Open	9:00am	5:00pm
Thursday	Open	9:00am	5:00pm
Friday	Open	9:00am	5:00pm
Saturday	Open	9:00am	5:00pm
Sunday	Open