Gene Knockout Cell Line Generation

Gene is a DNA or RNA sequence that codes for a molecule that has a function. A chromosome consists of a long strand of DNA containing many genes. For example, a human chromosome can have up to 500 million base pairs of DNA with thousands of genes. In genetic study, gene knockout or overexpression are widely applied for function study. Since the development of CRISPR system, it becomes easier to get a gene knockout cell line or model for further research. Using CRISPR/Cas9 for gene knockout, an indel is introduced to the target loci that results in a frame shift mutation. When applied for gene knockout, sgRNA is designed to target the exons of gene. Then Cas9 will be recruited to the specific loci and induce DSB. Indels occur when repairing DNA double strand break in error-prone way.

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