Introduction
Intracellular flow cytometry can be used to analyze a variety of intracellular molecules including cytokines, inflammatory mediators, transcription factors, and phosphoproteins. It can provide rich information concerning cellular function and signaling responses. Different from cell surface markers staining, intracellular antigens detection requires cell fixation and permeabilization before staining. Typically, cells are fixed with formaldehyde to preserve the cellular morphology, and then permeabilized with detergent or alcohol. Such fixation/permeabilization treatment allows the antibodies against intracellular antigens across the plasma membrane to stain intracellularly, while maintaining the morphological characteristics of cells.
Contact Info
Address:
45-1 Ramsey Road, Shirley, NY 11967, USA
Shirley
New York 11967
United States
Shirley
New York 11967
United States
Tel:
6316197922
Website:
https://www.creative-diagnostics.com/flow-cytometry-protocol-intracellular-staining.htm
Offering
- Biotechnology
Hours of operation
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