Introduction
Two-dimensional gel electrophoresis, abbreviated as 2-DE, is a powerful and widely used tool that uses gel electrophoresis to analyze mixtures of proteins. While both isoelectric focusing and SDS-PAGE are powerful techniques, 2D electrophoresis is a clever combination of the two methods. Proteins are first separated by their isoelectric point (pl) in isoelectric focusing, followed by a second separation through SDS-PAGE that resolves proteins based on molecular weight. Every protein spot on a 2D electrophoresis can be eluted and identified via high-throughput mass spectrometry. Therefore, 2D electrophoresis is particularly useful to compare protein profiles between different tissues, conditions, or between control and treated samples.
Contact Info
Address:
45-1 Ramsey Road, Shirley, NY 11967, USA
shirley
11967
United States
shirley
11967
United States
Tel:
Website:
https://www.creative-proteomics.com/services/2d-electrophoresis-2.htm
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