Protein expression
13 RESULTS
45-1Ramsey Road, Shirley
Tel: 5166698109
Profacgen is a state-of-the-art protein service provider located in Long Island NY USA. We provide custom protein services in the biological sciences enabling access to the latest tools techniques and expertise with competitive pricing and rapid turnaround time. We serve a broad spectrum of industrial and academic clients with a commitment to delivering high-quality data and customer services.... read more
Tel: 5166698109
Profacgen is a state-of-the-art protein service provider located in Long Island NY USA. We provide custom protein services in the biological sciences enabling access to the latest tools techniques and expertise with competitive pricing and rapid turnaround time. We serve a broad spectrum of industrial and academic clients with a commitment to delivering high-quality data and customer services.... read more
45-1 Ramsey Road, Shirley, New York 11967, USA, Shirley
At Profacgen we use lentiviral vector for efficient gene delivery and to achieve high integration rate. Limited dilution and antibiotics screening are then applied to select for high-yield genetically homogenous stable expression monoclones. When fluorescent protein is fused to the target protein we can also use flow cytometry to screen for positive clones. Profacgen also offers the generation of Tet-ON inducible stable cell lines for inducible expression of your target gene. Doxycycline can then be used to induce the expression of your target gene... read more
At Profacgen we use lentiviral vector for efficient gene delivery and to achieve high integration rate. Limited dilution and antibiotics screening are then applied to select for high-yield genetically homogenous stable expression monoclones. When fluorescent protein is fused to the target protein we can also use flow cytometry to screen for positive clones. Profacgen also offers the generation of Tet-ON inducible stable cell lines for inducible expression of your target gene. Doxycycline can then be used to induce the expression of your target gene... read more
45-1 Ramsey Road, Shirley, NY 11967, USA, Shirley
Tel: 6316197922
Profacgen has organized a team of protein biologists who have extensive experience in protein expression to perform thorough feasibility analysis before the initiation of each protein expression project. We will evaluate the possibility of soluble expression secretory expression correct modification and folding production efficiency necessity of purification steps and protein buffer formulation etc.... read more
Tel: 6316197922
Profacgen has organized a team of protein biologists who have extensive experience in protein expression to perform thorough feasibility analysis before the initiation of each protein expression project. We will evaluate the possibility of soluble expression secretory expression correct modification and folding production efficiency necessity of purification steps and protein buffer formulation etc.... read more
45-1 Ramsey Road, Shirley, New York 11967, USA, Shirley
Recombinant protein expression is the technique of target protein production using the recombinant DNA technology. It is now commonly used in bioresearch and in pharmaceutical production of recombinant protein therapeutics. The recombinant DNA usually the cDNA sequence of the target protein is designed to be under the control of a well characterized promoter and express the target protein within the chosen host cell to achieve high-level protein expression.... read more
Recombinant protein expression is the technique of target protein production using the recombinant DNA technology. It is now commonly used in bioresearch and in pharmaceutical production of recombinant protein therapeutics. The recombinant DNA usually the cDNA sequence of the target protein is designed to be under the control of a well characterized promoter and express the target protein within the chosen host cell to achieve high-level protein expression.... read more
45-1 Ramsey Road, Shirley, New York 11967, USA, Shirley
Tel: 1-631-619-7922
Creative BioMart is committed to providing advanced tools for protein expression and purification. As a leading supplier for reagents in the biotechnology field we understand the importance of convenient and easy-to-use systems for high level expression and sample purification. We invite you to review our growing range of expression systems resulting from our experience in cloning overexpression and purification. http://www.creativebiomart.net/Protein-Expression-Services.htm... read more
Tel: 1-631-619-7922
Creative BioMart is committed to providing advanced tools for protein expression and purification. As a leading supplier for reagents in the biotechnology field we understand the importance of convenient and easy-to-use systems for high level expression and sample purification. We invite you to review our growing range of expression systems resulting from our experience in cloning overexpression and purification. http://www.creativebiomart.net/Protein-Expression-Services.htm... read more
45-1 Ramsey Road, Shirley, New York 11967, USA, Shirley
Profacgen provides several approaches for protein co-expression service. The easiest is to have multiple genes encoded by one vector or virus and co-transform or co-infect the host with several of them. Alternatively each gene is encoded by a single vector or virus either under the control of a single promoter or of several promoters and a single transformation or infection is required. Profacgen offers unprecedented expertise and high quality custom co-expression service with competitive pricing. Request more information to see how Profacgen could be assistance.... read more
Profacgen provides several approaches for protein co-expression service. The easiest is to have multiple genes encoded by one vector or virus and co-transform or co-infect the host with several of them. Alternatively each gene is encoded by a single vector or virus either under the control of a single promoter or of several promoters and a single transformation or infection is required. Profacgen offers unprecedented expertise and high quality custom co-expression service with competitive pricing. Request more information to see how Profacgen could be assistance.... read more
45-1 Ramsey Road, Shirley
Tel: 16313721052Â
Proteins are the building blocks of all living organisms and play crucial roles in biological processes. To investigate how a particular protein modulates biological processes many aspects of the protein such as structure modification localization interaction and function needs to be studied. A considerable number of proteins especially membrane proteins are screened in drug discovery as biological targets or as potential drugs themselves. Most of these proteins are produced using cell-based or cell-free expression systems which typically includes the transcription of recombinant DNA into mRNA that is further translated into the protein under study. Creative Biostructure uses both cell-based and... read more
Tel: 16313721052Â
Proteins are the building blocks of all living organisms and play crucial roles in biological processes. To investigate how a particular protein modulates biological processes many aspects of the protein such as structure modification localization interaction and function needs to be studied. A considerable number of proteins especially membrane proteins are screened in drug discovery as biological targets or as potential drugs themselves. Most of these proteins are produced using cell-based or cell-free expression systems which typically includes the transcription of recombinant DNA into mRNA that is further translated into the protein under study. Creative Biostructure uses both cell-based and... read more
45-1 Ramsey Road, Shirley, NY 11967, USA, Shirley
Tel: 631 619 7922
Cell-free expression system produces proteins in vitro through translation machineries extracted from cells. It has become a well-developed technique for synthesizing proteins in cell lysates. This approach meets with the increasing demands for a more straightforward rapid and efficient way of protein production. Cell-free expression overcomes many obstacles of conventional cellular expression. Firstly it is ideal for high-throughput screening of mutagenesis. Moreover the system works independently and is free of cellular context. The whole machinery sets up a better environment to manufacture toxic proteins membrane proteins and proteins that would be easily degraded by intracellular proteases. In addition the reaction... read more
Tel: 631 619 7922
Cell-free expression system produces proteins in vitro through translation machineries extracted from cells. It has become a well-developed technique for synthesizing proteins in cell lysates. This approach meets with the increasing demands for a more straightforward rapid and efficient way of protein production. Cell-free expression overcomes many obstacles of conventional cellular expression. Firstly it is ideal for high-throughput screening of mutagenesis. Moreover the system works independently and is free of cellular context. The whole machinery sets up a better environment to manufacture toxic proteins membrane proteins and proteins that would be easily degraded by intracellular proteases. In addition the reaction... read more
45-16 Ramsey Road, Shirley, NY 11967, US, Shirley
Proteins are the building blocks of all living organisms and play crucial roles in biological processes. To investigate how a particular protein modulates biological processes many aspects of the protein such as structure modification localization interaction and function needs to be studied. A considerable number of proteins especially membrane proteins are screened in drug discovery as biological targets or as potential drugs themselves. Most of these proteins are produced using cell-based or cell-free expression systems which typically includes the transcription of recombinant DNA into mRNA that is further translated into the protein under study.... read more
Proteins are the building blocks of all living organisms and play crucial roles in biological processes. To investigate how a particular protein modulates biological processes many aspects of the protein such as structure modification localization interaction and function needs to be studied. A considerable number of proteins especially membrane proteins are screened in drug discovery as biological targets or as potential drugs themselves. Most of these proteins are produced using cell-based or cell-free expression systems which typically includes the transcription of recombinant DNA into mRNA that is further translated into the protein under study.... read more
45-1 Ramsey Road, Shirley, New York 11967, USA, Shirley
Profacgen has investigated the use of E. coli co-expression techniques in protein manufacturing and tried to extend from the common binary expression to the more complicated multi-expression. Results from various case studies have indicated co-expression in E. coli host to be a good alternative compared to endogenous purification or in vitro reconstruction from individually expressed components. It is also proved that co-expression of multiple proteins/complexes a useful alternative to the complicated and expensive expression systems such as yeast baculovirus or mammalian cell culture.... read more
Profacgen has investigated the use of E. coli co-expression techniques in protein manufacturing and tried to extend from the common binary expression to the more complicated multi-expression. Results from various case studies have indicated co-expression in E. coli host to be a good alternative compared to endogenous purification or in vitro reconstruction from individually expressed components. It is also proved that co-expression of multiple proteins/complexes a useful alternative to the complicated and expensive expression systems such as yeast baculovirus or mammalian cell culture.... read more
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