IF Protocol Free Floating Section
2 RESULTS
45-1 Ramsey Road, Shirley, NY 11967, USA, Shirley
Tel: 6316197922
Reagents: PBS: To prepare 1 L 1X PBS: dissolve 8g of NaCl 0.2g of KCl 1.44g of Na2HPO4 0.24g of KH2PO4 in 800ml distilled H2O then adjust pH to 7.4 with HCl and adjust volume to 1L with additional distilled H2O. Permeablization solution: 0.3% Triton X-100 in PBS. To prepare 100 mL: add 300 µl Triton X-100 to 100 mL PBS and mix. (Optional) Blocking buffer: To prepare 10 ml add 0.5 ml normal serum in PBS and mix well. While stirring add 30 µl Triton™ X-100. NOTE: Select serum that are from the same species as the secondary antibody.... read more
Tel: 6316197922
Reagents: PBS: To prepare 1 L 1X PBS: dissolve 8g of NaCl 0.2g of KCl 1.44g of Na2HPO4 0.24g of KH2PO4 in 800ml distilled H2O then adjust pH to 7.4 with HCl and adjust volume to 1L with additional distilled H2O. Permeablization solution: 0.3% Triton X-100 in PBS. To prepare 100 mL: add 300 µl Triton X-100 to 100 mL PBS and mix. (Optional) Blocking buffer: To prepare 10 ml add 0.5 ml normal serum in PBS and mix well. While stirring add 30 µl Triton™ X-100. NOTE: Select serum that are from the same species as the secondary antibody.... read more
45-1 Ramsey Road, Shirley, NY 11967, USA, Shirley
Tel: 6316197922
This protocol provides a detailed procedure for immunofluorescence on free floating sections from tissue sample.... read more
Tel: 6316197922
This protocol provides a detailed procedure for immunofluorescence on free floating sections from tissue sample.... read more
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