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45-1 Ramsey Road, Shirley, New York 11967, USA, Shirley
Rosemary (Rosmarinus officinalis) is a woody perennial spice plant native to the Mediterranean basin and grown in chalky soil. Aromatic oil can be extracted from stems leaves and flowers of rosemary. The varieties of rosemary can be divided into two types according to the shape of the plant one is the vertical type with trunk growing upward the other is the creeping type with branches growing laterally. The name “rosemary†has nothing to do with the “rose†and “Maryâ€. It is derived from the Latin name “rosmarinus†composed of the roots of “dew†(ros) and “sea†(marinus) which means “dew... read more
Rosemary (Rosmarinus officinalis) is a woody perennial spice plant native to the Mediterranean basin and grown in chalky soil. Aromatic oil can be extracted from stems leaves and flowers of rosemary. The varieties of rosemary can be divided into two types according to the shape of the plant one is the vertical type with trunk growing upward the other is the creeping type with branches growing laterally. The name “rosemary†has nothing to do with the “rose†and “Maryâ€. It is derived from the Latin name “rosmarinus†composed of the roots of “dew†(ros) and “sea†(marinus) which means “dew... read more
45-1 Ramsey Road, Shirley, New York 11967, USA, Shirley
Purple potato is also known as black potato and its tuber flesh has a purple to dark purple color. In addition to the nutrients of ordinary potatoes it is also rich in selenium and anthocyanins. Anthocyanins have a preventive and therapeutic effect on more than 100 diseases and are known as the seventh most important nutrient after water protein fat carbohydrates vitamins and minerals. Anthocyanins are the most direct effective and safe free radical scavengers found in the scientific community to prevent diseases and maintain human health. The ability to scavenge free radicals is 20 times that of vitamin C... read more
Purple potato is also known as black potato and its tuber flesh has a purple to dark purple color. In addition to the nutrients of ordinary potatoes it is also rich in selenium and anthocyanins. Anthocyanins have a preventive and therapeutic effect on more than 100 diseases and are known as the seventh most important nutrient after water protein fat carbohydrates vitamins and minerals. Anthocyanins are the most direct effective and safe free radical scavengers found in the scientific community to prevent diseases and maintain human health. The ability to scavenge free radicals is 20 times that of vitamin C... read more
45-1 Ramsey Road, Shirley, New York 11967, USA, Shirley
Substrate binding has additional effects that enhance reaction rates among which proximity and orientation gives the most obvious increase. There are two characteristics of enzyme-substrate binding where the first one is that the binding brings together substrates and the reactive groups on the active site of the enzyme. The second one is that active site in the enzyme is extremely specific in the substrates therefore making the reaction as efficient as possible. Proximity and orientation in enzyme-substrate interactions could align the reactive chemical groups and make them come together in an optimal orientation with the proper spatial relationship for a... read more
Substrate binding has additional effects that enhance reaction rates among which proximity and orientation gives the most obvious increase. There are two characteristics of enzyme-substrate binding where the first one is that the binding brings together substrates and the reactive groups on the active site of the enzyme. The second one is that active site in the enzyme is extremely specific in the substrates therefore making the reaction as efficient as possible. Proximity and orientation in enzyme-substrate interactions could align the reactive chemical groups and make them come together in an optimal orientation with the proper spatial relationship for a... read more
45-1 Ramsey Road, Shirley, New York 11967, USA, Shirley
Protein pull down assay is an in vitro affinity purification method that uses a bait protein to enrich proteins that interact with the bait protein. It can be used for confirmation of existing protein-protein interactions discovered by other techniques or initial screening to identify novel protein-protein interactions. Profacgen provides you protein pull down service for the detection of possible interacting proteins of your target proteins. The basic principle of pull down assay is to utilize a tag fused protein (such as GST-tag His-tag and biotin-tag) immobilized to affinity resin as the bait protein. Proteins binding to the bait protein (prey... read more
Protein pull down assay is an in vitro affinity purification method that uses a bait protein to enrich proteins that interact with the bait protein. It can be used for confirmation of existing protein-protein interactions discovered by other techniques or initial screening to identify novel protein-protein interactions. Profacgen provides you protein pull down service for the detection of possible interacting proteins of your target proteins. The basic principle of pull down assay is to utilize a tag fused protein (such as GST-tag His-tag and biotin-tag) immobilized to affinity resin as the bait protein. Proteins binding to the bait protein (prey... read more
45-1 Ramsey Road, Shirley, New York 11967, USA, Shirley
Co-IP is a classic technology widely used for protein-protein interaction identification and validation. Based on the specific immunological interaction between the bait protein and its antibody co-IP has become an effective and reliable method in detecting the physiological interaction between proteins. The principle of the co-IP technique is as follows: Many intracellular protein-protein interactions are retained when cells are lysed under non-denaturing conditions. The bait protein for example protein X can be captured by its specific antibody stabilized to agarose beads. If there is another protein the prey protein for example protein Y binds to protein X in vivo the... read more
Co-IP is a classic technology widely used for protein-protein interaction identification and validation. Based on the specific immunological interaction between the bait protein and its antibody co-IP has become an effective and reliable method in detecting the physiological interaction between proteins. The principle of the co-IP technique is as follows: Many intracellular protein-protein interactions are retained when cells are lysed under non-denaturing conditions. The bait protein for example protein X can be captured by its specific antibody stabilized to agarose beads. If there is another protein the prey protein for example protein Y binds to protein X in vivo the... read more
45-1 Ramsey Road, Shirley, New York 11967, USA, Shirley
Profacgen provides a comprehensive panel of services for the study of protein-protein interactions of which the Surface Plasmon Resonance (SPR) analysis is commonly used by our customers for the highly accurate and sensitive kinetics and affinity measurement. For SPR analysis the ligand-of-interest is immobilized on the surface of the biosensor chip while the analyte is injected to flow through the chip surface. Dynamic change of the SPR angle can be detected and assayed for binding affinity when interaction happens between the ligand and the analyte.... read more
Profacgen provides a comprehensive panel of services for the study of protein-protein interactions of which the Surface Plasmon Resonance (SPR) analysis is commonly used by our customers for the highly accurate and sensitive kinetics and affinity measurement. For SPR analysis the ligand-of-interest is immobilized on the surface of the biosensor chip while the analyte is injected to flow through the chip surface. Dynamic change of the SPR angle can be detected and assayed for binding affinity when interaction happens between the ligand and the analyte.... read more
45-1 Ramsey Road, Shirley, New York 11967, USA, Shirley
The MbY2H system is also called the split ubiquitin Y2H whereas your bait protein will be fused to the C-terminal half of ubiquitin (Cub) and a transcription factor; and the transmembrane bait protein library is fused to the N-terminal half (Nub). Upon association of the bait and prey a compete ubiquitin molecule is formed and cleaved by endogenous protease releasing the transcription factor to initiate the transcription of the reporter genes. The most widely used reporter genes are the same as regular Y2H systems including His3 and LacZ. Taking advantage of Profacgen’s MbY2H system and comprehensive membrane cDNA libraries from... read more
The MbY2H system is also called the split ubiquitin Y2H whereas your bait protein will be fused to the C-terminal half of ubiquitin (Cub) and a transcription factor; and the transmembrane bait protein library is fused to the N-terminal half (Nub). Upon association of the bait and prey a compete ubiquitin molecule is formed and cleaved by endogenous protease releasing the transcription factor to initiate the transcription of the reporter genes. The most widely used reporter genes are the same as regular Y2H systems including His3 and LacZ. Taking advantage of Profacgen’s MbY2H system and comprehensive membrane cDNA libraries from... read more
45-1 Ramsey Road, Shirley, New York 11967, USA, Shirley
Profacgen offers a wide range of ready-to-use prey protein cDNA libraries. We also can custom develop homogenized cDNA libraries according to your specific requirements. Screening and validation process can be done in as short as two months! We also provide co-immunoprecipitation service to validate your result from the Y2H screening.... read more
Profacgen offers a wide range of ready-to-use prey protein cDNA libraries. We also can custom develop homogenized cDNA libraries according to your specific requirements. Screening and validation process can be done in as short as two months! We also provide co-immunoprecipitation service to validate your result from the Y2H screening.... read more
45-1 Ramsey Road, Shirley, New York 11967, USA, Shirley
Precise mutagenesis of a target protein is very useful in the study of protein-protein interaction protein structure biological activity site etc. Utilizing the most advanced DNA synthesis technology Profacgen provides one-stop service on Site-Directed Protein Mutagenesis Service. Single or multiple mutations (substitution insertion or deletion) can be introduced into your target protein with high accuracy and efficiency. We can also create protein truncation according to your specific requirements.... read more
Precise mutagenesis of a target protein is very useful in the study of protein-protein interaction protein structure biological activity site etc. Utilizing the most advanced DNA synthesis technology Profacgen provides one-stop service on Site-Directed Protein Mutagenesis Service. Single or multiple mutations (substitution insertion or deletion) can be introduced into your target protein with high accuracy and efficiency. We can also create protein truncation according to your specific requirements.... read more
45-1 Ramsey Road, Shirley, New York 11967, USA, Shirley
Protein mutant libraries containing different protein variants are becoming more and more popular in the determination of vital residues controlling physiological activities and pathological process. Novel components of signal pathways or receptors can be identified through screening of protein mutant libraries. Profacgen's high-throughput gene synthesis mutagenesis and protein production platforms enable us to speed up your project by offering protein mutant library construction service which can deliver thousands of protein variants efficiently. Our protein mutant library construction service consists of gene synthesis mutagenesis sub-cloning pilot scale protein expression using the E. coli system and final protein purification.... read more
Protein mutant libraries containing different protein variants are becoming more and more popular in the determination of vital residues controlling physiological activities and pathological process. Novel components of signal pathways or receptors can be identified through screening of protein mutant libraries. Profacgen's high-throughput gene synthesis mutagenesis and protein production platforms enable us to speed up your project by offering protein mutant library construction service which can deliver thousands of protein variants efficiently. Our protein mutant library construction service consists of gene synthesis mutagenesis sub-cloning pilot scale protein expression using the E. coli system and final protein purification.... read more
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